Coding

Part:BBa_K1039009:Design

Designed by: Urooj Kishor   Group: iGEM13_Waterloo   (2013-09-12)


PhiC31 Invertible Promoter Switch (Promoter J23118) – PB State


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3
    Illegal NheI site found at 189
    Illegal NheI site found at 212
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The genes on the prefix side of the switch needed to be inverted.


Source

The DNA was synthesized through New England BioLabs

References